[Publication date of latest article cited: October 16, 2023]<\/p>\n\n\n\n
Saliva and mucous, and the tissues producing them, are some of the main routes in which SARS-CoV-2 replicates and transmits (Collins<\/a>; Huang N, Perez, et al.<\/a>; Li H, Wang Y, et al.<\/a>; Nazaroff<\/a>; Wang C, Prather K, et al.<\/a>). SARS-CoV-2 replicates in the throat (W\u00f6lfel et al.<\/a>), mouth, tongue, and salivary glands because they have much angiotensin-converting enzyme 2 (ACE2), transmembrane protease serine 2 (TMPRSS2), and furin. Those viruses attach to those molecules to enter cells (Huang N, P\u00e9rez, et al.<\/a>; Salamanna et al.<\/a>; Salas Orozco et al.<\/a>; Xu H, Zhong L et al.<\/a>; Xu J, Li Y et al.<\/a>). PCR tests found SARS-CoV-2 RNA in saliva samples and oral swabs of patients (Abasiyanik et al.<\/a>; Chen W, Lan, et al.<\/a>; Huang N, Perez, et al.<\/a>; Karia et al.<\/a>; To KKW, Tsang, Leung et al.<\/a>; To KKW, Tsang, Yip et al.<\/a>; W\u00f6lfel et al.<\/a>; Zhang W, Du et al.<\/a>; Zhou J, Singanayagam A, et al.<\/a>), showing that saliva or mucous can probably transmit the novel coronavirus. Tests using viral cultures showed that some patients shed viable viruses for up to 60 days (Arons et al.<\/a>; Huang N, Perez, et al.<\/a>; Karia et al.<\/a>; Kujawski et al.<\/a>; W\u00f6lfel et al<\/a>.; Zhou J, Singanayagam A, et al.<\/a>). SARS-CoV-2 viral load (the number of viruses) peaks at about the fifth day after start of symptoms, lasting on average 17 days. But infected people did not infect another person more than 9 days after symptom onset (Cevik, Tate, et al.<\/a>; Coleman<\/a>). Cases with higher viral loads were more likely to transmit to secondary cases, with shorter incubation periods in the secondary cases (Marks et al.<\/a>).<\/p>\n\n\n\n Exhaling, speaking, sneezing, or coughing spreads saliva droplets of different sizes into the air, that can go in the mouth, nose, or eyes of another person (Anfinrud et al.<\/a>; Asadi, Wexler, et al.<\/a>; Nazaroff<\/a>; Wang C, Prather K, et al.<\/a>). Pushing air through the bronchioles, main bronchi, trachea, larynx, vocal cords, mouth, tongue, and lips expels these droplets and particles (Nazaroff<\/a>). The larger droplets (>100 \u03bcm, microns, micrometers in diameter) usually float less than 2 meters in distance (6 feet) and fall down onto a surface. Aerosols <100 \u03bcm can float >5 seconds, farther than 2 meters distance, and enter a person\u2019s respiratory tract (Nazaroff<\/a>; Wang C, Prather K, et al.<\/a>). The numbers of droplets and aerosols peaks in the first 5 seconds and decreased numbers linger afterward (Asai et al.<\/a>) Most aerosols are <5 \u03bcm, so they can carry millions of SARS-CoV-2 to other people in the room. When a person inhales them, they can deposit in and infect the mucous membranes (Atkinson et al.<\/a>; Brosseau<\/a>; Centers for Disease Control and Prevention \u201cScientific Brief: SARS-CoV-2 and Potential Airborne Transmission\u201d<\/a>; Centers for Disease Control and Prevention \u201cScientific Brief: SARS-CoV-2 Transmission\u201d<\/a>; Dhand, Li<\/a>; Jones N, Qureshi et al.<\/a>; Lerner et al<\/a>; Meselson<\/a>; Nazaroff<\/a>; Tang, Li et al.<\/a>; Wang C, Prather K, et al.<\/a>; WHO \u201cCoronavirus disease (COVID-19): How is it transmitted?\u201d<\/a>).<\/p>\n\n\n\n The more loudly and forcefully a person talks or sings, the more aerosol particles they produce. Singing produces more aerosols than talking (Alsved et al.<\/a>; Asadi, Wexler, et al.; Jones N, Qureshi et al.<\/a>).<\/p>\n\n\n\n Respiratory droplets and close contact probably cause much of the COVID-19 transmission (Centers for Disease Control and Prevention \u201cScientific Brief: SARS-CoV-2 and Potential Airborne Transmission<\/a>; Nazaroff<\/a>; Wang C, Prather K, et al.<\/a>; Wiersinga et al.<\/a>; World Health Organization-China<\/a>). Some German medical scientists who examined novel coronavirus RNA from their patients also think that it is spread mostly by droplets in the air (W\u00f6lfel et al<\/a>). Hong Kong scientists found SARS-CoV-2 replicates competently in bronchus and lung cells (Hui et al.<\/a>). Infected people of all ages have the viruses in their nasopharynx (nose and throat), but small children have more of the viral RNA, indicating (but not completely proving) that they might transmit to others more than older children and adults transmit (Heald-Sargent et al.<\/a>).<\/p>\n\n\n\n Using mouthwashes could damage SARS-CoV-2 lipid envelopes, and thus prevent some people from getting infected or infecting others with COVID-19 (Chumpitaz-Cerrate et al.<\/a>; Huzar, Flynn<\/a>; O\u2019Donnell et al.<\/a>; Stone<\/a>). Scientists tested common over-the-counter mouthwashes in artificial in vitro laboratory conditions that mimic the nasopharyngeal secretions in people\u2019s mouths. A systematic review found that only chlorhexidine made a significant difference in PCR-detected viral load compared to controls (Sbricoli et al.<\/a>) Other studies They found that mouthwashes containing ethanol, polyvidone iodine, dequalinium chloride, and benzalkonium chloride inactivated almost all the SARS-CoV-2 (Chiu \u201cWhat those studies on mouthwash\u201d<\/a>; Meister et al.<\/a>). Other studies showed that some over-the-counter nasal rinses and mouthwashes containing cetylpyridinium chloride (Filippo et al.<\/a>), methyl, thymol, and povidone iodine inactivated the coronaviruses that cause common colds (Meyers et al.<\/a>). A controlled clinical trial in COVID-19 patients found that \u00df-cyclodextrin and citrox (bioflavonoids) (CDCM) mouthwashes reduced SARS-CoV-2 viral load modestly compared to a placebo (Carrouel et al.<\/a>). Chlorhexidine reduced SARS-CoV-2 also, in some experiments (Rahman GS et al.<\/a>) as well as cetylpyridinium chloride, methyl, thymol, and povidone iodine, and in other experiments not as well (Dami\u00e3o Costa et al.<\/a>; Fernandez et al.<\/a>). Scientists should do more research on people using these methods (Chumpitaz-Cerrate et al.<\/a>). But people should not over-interpret this to mean that these products will protect or cure most people from COVID-19 (Wu \u201cNo, Mouthwash will not save you\u201d<\/a>). If someone frequently gargled those mouthwashes, and aerosols with coronaviruses floated into their mouth and landed on a surface with mouthwash on it, that could destroy most of those coronaviruses. But many other coronaviruses could still float through their nose and mouth into their respiratory system and infect the mouthwash user.<\/p>\n\n\n\n Since some mouthwashes destroy coronaviruses in the mouth, people should not use them for hours before using saliva in a PCR or antigen test for SARS-CoV-2. The mouthwash could cause a false negative test (Lippi et al.<\/a>).<\/p>\n\n\n\n [Publication date of latest article cited: October 16, 2023]<\/p>\n\n\n\n Airborne spread of smaller droplets (called aerosols) is probably the main transmission mode of SARS-CoV-2 (Atiyani et al.<\/a>; Centers for Disease Control and Prevention \u201dScientific Brief: SARS-CoV-2 and Potential Airborne Transmission\u201d<\/a>; \u201cScientific Brief: SARS-CoV-2 Transmission\u201d<\/a>; \u201cHow COVID-19 Spreads\u201d<\/a>; Greenhalgh et al. \u201cTen Scientific Reasons\u201d<\/a>; Harvey A, Fuhrmeister, et al.<\/a>; Khamsi<\/a>; Lee, Wada, et al.<\/a>; Lerner et al.<\/a>; Mandavilli \u201c239 experts\u201d<\/a>; Morawska, Milton<\/a>; Morrow et al.<\/a>; National Academies \u201cAirborne Transmission of SARS-CoV-2\u201d<\/a>; Nazaroff<\/a>; Tang J, Marr, et al.<\/a>; Tang S, Mao<\/a>; The Lancet Respiratory Medicine<\/a> ;Wang C, Prather K, et al.<\/a>; WHO \u201cCoronavirus disease (COVID-19): How is it transmitted?\u201d<\/a>; Xu C, Liu W, et al.<\/a>; Zhang R, Li Y, et al.<\/a>). But this is difficult to prove because most transmission situations have a mixture of routes: some droplets are flying and becoming aerosols, or falling on fomite surfaces, which dry into dust that floats up from surfaces into air (Klompas et al.<\/a>; National Academies \u201cAirborne Transmission of SARS-CoV-2\u201d<\/a>; WHO \u201cTransmission of SARS-CoV-2\u201d<\/a>; Nazaroff<\/a>; WHO \u201cAdvice on the Use of Masks\u201d<\/a>; Xu C, Liu W, et al.<\/a>; Zhou J, Singanayagam A, et al.<\/a>). In some experiments, scientists controlled the variables, and hamsters did transmit SARS-CoV-2 to other hamsters through air (Chan J, Yuan et al.<\/a>; Gandhi, Marr<\/a>; Medical Xpress<\/a>; Mohandas et al.<\/a>). But, the varied research and calculation methods in most studies, and the many studies trying to culture from air samples and not succeeding \u201cprevents firm conclusions over airborne transmission\u201d (Heneghan, Spencer, et al.<\/a>). The evidence, however, is sufficient to wear masks, stay physically distant or outdoors, avoid crowding, ventilate, and use air filters, to protect ourselves.<\/p>\n\n\n\n Aerosol transmission occurs when coughing, talking, and breathing produce turbulent gas clouds with large and small droplets. Those larger than 100 micrometers usually fall in seconds, within 2 meters. But smaller droplets, called airborne aerosols, can float for hours, dry out with viruses still viable in them, and dissipate many meters, even into other rooms. But this varies with air conditions; larger droplets sometimes float, and small aerosols can drop (Bourouiba<\/a>; Bromage<\/a>; Brosseau<\/a>; Jones N, Qureshi et al.<\/a>; National Academies \u201cAirborne Transmission of SARS-CoV-2\u201d<\/a>; Nazaroff<\/a>; Stadnytskyi et al.<\/a>; Tang J, Marr, et al.<\/a>; Wang C, Prather K, et al.<\/a>). The numbers of viruses start high when they are first aerosolized, and decrease as the droplets evaporate and float away. This explains why people should stay distant from each other to reduce the probability of inhaling large numbers of SARS-CoV-2 (Asa<\/a>i et al.<\/a>; Dabisch et al.<\/a>). When inhaled, they can float past the cilia and mucous membranes, and go into the lungs and infect the alveoli air sacs (Meselson<\/a>; Wang C, Prather K, et al.<\/a>).<\/p>\n\n\n\n COVID-19 infected people exhale millions of SARS-CoV-2 viruses just by ordinary breathing, especially during early stages (Ma et al.<\/a>; Wang C, Prather K, et al.<\/a>). SARS-CoV-2 was found in the air near them days after symptoms onset (Adenaiye et al.)<\/a>. Likewise, in a Florida hospital, viable SARS-CoV-2 viruses were collected from air 7 feet and 16 feet from COVID-19 patients, and cultured in cells, showing they could potentially infect other people (Lednicky et al.<\/a>; Mandavilli \u2018A Smoking Gun\u2019<\/a>). In a Nebraska hospital, some air samples in COVID-19 patients\u2019 rooms and in hallways outside rooms had SARS-CoV-2 RNA, even near patients who were not coughing. Some samples could be cultured, and virions seen in transmission electron microscopy (TEM). So, it may be possible to get infected from inhaling air in those rooms and hallways (Santarpia, Herrera, et al.<\/a>; Santarpia, Rivera, et al.<\/a>). In a Singapore hospital, PCR found SARS-CoV-2 RNA in the air of most patients\u2019 rooms, in particles sized >4\u2009\u00b5m and 1\u20134\u2009\u00b5m, even though none had aerosol-generating procedures or intranasal oxygen supplementation (Chia et al.<\/a>). In London hospitals, RT-qPCR found SARS-CoV-2 RNA in the air in patients\u2019 room, staff rooms, and a public area. They could not culture the viruses, perhaps because of low concentrations or long times since viruses were left (Zhou J, Otter, et al.<\/a>). Even 12-47 days after symptom onset, air samples around patients had SARS-CoV-2 RNA (Feng B, Xu K, et al.<\/a>).<\/p>\n\n\n\n In Wuhan hospitals scientists found SARS-CoV-2 RNA in air samples in many rooms, with different kinds of patients, especially those having little ventilation (Guo et al.<\/a>). Scientists found the RNA in the air of bathrooms, which patients used only a few minutes at a time. Perhaps the patients coughed the viruses into the air, and closed the bathroom door, leaving the tiny aerosol droplets to float for hours. But the air in critical care unit (CCU), intensive care unit (ICU), ward room, and workstations had no or low concentrations of SARS-CoV-2 RNA, possibly because they exchanged air at high rates or were using negative pressure ventilation (Liu, Ning et al.<\/a>; Ong et al.<\/a>). Aerosols exhaled by patients float and spread all round a room (Saw et al.<\/a>).<\/p>\n\n\n\n Scientists also found the RNA in the air outside medical centers, where outpatients and others went, some of whom could be asymptomatic carriers (Amato-Louren\u00e7o et al.<\/a>; Liu, Ning, et al.<\/a>).<\/p>\n\n\n\n Finding SARS-CoV-2 RNA in the air still did not prove that it was actually transmitted through the air. But, an experiment showed it could be transmitted through over a meter of air around corners, from infected ferrets to other ferrets. The large droplets probably did not travel that distance, proving that tiny aerosols probably transmitted the viruses (Kozlov<\/a>; Kutter et al.<\/a>).<\/p>\n\n\n\n Aerosol transmission in air flowing around corners probably occurred in an apartment building through vertical air ducts connecting bathrooms. In a large, multi-wing building, only people in rooms sharing the same ducts were infected, and were probably not infected by other sources (Hwang et al.<\/a>).<\/p>\n\n\n\n SARS-CoV-2 could float through aerosols, land on fomite surfaces, and stay there (Nazaroff<\/a>; Wang C, Prather K, et al.<\/a>). For example, these viruses were on 65% of surfaces near infected people (Adenaiye et al.<\/a>). From an apartment with COVID-19 infected people, SARS-CoV-2 was found on surfaces in the unoccupied room upstairs. The only apparent connection was through a bathroom drain pipe (Laguipo<\/a>; Tang S, Mao et al<\/a>.).<\/p>\n\n\n\n SARS-CoV-2 can also float from surfaces up into the air (National Academies \u201cAirborne Transmission of SARS-CoV-2\u201d<\/a>; Nazaroff<\/a>). In Wuhan hospitals, the rooms where medical staff changed into and out of personal protective equipment (PPE) clothing had the virus RNA in the air. Maybe just moving the clothes and PPE around shook viruses into the air (Liu, Ning et al.)<\/a> .<\/p>\n\n\n\n Scientists conducted lab experiments demonstrating that SARS-CoV-2 can float in air for hours and remain infectious (Nazaroff<\/a>; Wang C, Prather K, et al.<\/a>). In one, they nebulized SARS-CoV-2 into aerosols, took samples from the air three hours later, put these into living cells, and could culture viable viruses (van Doremalen et al.<\/a>). In another, they nebulized SARS-CoV-2 for up to 16 hours, and samples from that air showed little decrease in virus quantities, and no changes in virus morphology (Fears et al.<\/a>). These lab studies proved that airborne transmission is possible, but did not prove that it actually occurred out in society (Brosseau<\/a>; National Institutes of Allergies and Infectious Diseases<\/a>; van Doremalen et al.<\/a>).<\/p>\n\n\n\n Air conditions affect how long SARS-CoV-2 lasts (Nazaroff<\/a>; Wang C, Prather K, et al.<\/a>). Experiments found that sunlight inactivates SARS-CoV-2 in minutes (Department of Homeland Security “S&T’s Research”<\/a>; Schuit et al.<\/a>). Other experiments and epidemiology studies in different environments showed that SARS-CoV-2 lasted longer in cooler, drier air (Cooney et al.<\/a>). But this might affect transmission less than non-environmental factors. So, warm, humid weather might decrease transmission a little (Ahlawat et al.<\/a>; Department of Homeland Security “S&T’s Research”<\/a>; National Academies of Science “SARS-CoV-2 survival…”<\/a>; Science Daily<\/a>; Ward et al.<\/a>).<\/p>\n\n\n\n The menu sections under “Risky situations” explain more about how people transmitted via aerosols indoors and in airplanes, buses, and cars.<\/p>\n\n\n\n [Publication date of latest article cited: September 22, 2023]<\/p>\n\n\n\n SARS-CoV-2 can replicate in intestines (Qian Q, Fan, et al.<\/a>). Its RNA has been found in patients\u2019 feces and in anal swabs for weeks (Cevik, Tate, et al.<\/a>; Cuicchi et al.<\/a>; Jones DL et al.<\/a>; Karia et al.<\/a>; Li H, Wang Y, et al.<\/a>; Wang X, Zhou Y, et al.<\/a>; Xiao F, Tang M, et al<\/a>.; Zhang W, Du RH, et al.<\/a>). ACE2, the receptor chemical that SARS-CoV-2 uses to enter cells, is expressed in intestinal epithelial cells (An et al.<\/a>; Hamming et al.<\/a>; Salamanna et al.<\/a>; Xiao F, Tang M, et al.<\/a>), so SARS-CoV-2 replicates in the intestine (Amirian<\/a>; Lamers et al.<\/a>). Viable viruses have been cultured from stool (Chen W et al.<\/a>; Heneghan, Spencer \u201cSARS-CoV-2 and the role of orofecal transmission\u201d<\/a>; Su et al.<\/a>; Tang A et al.<\/a>; Wong et al.<\/a>; World Health Organization-China<\/a>; Xiao F, Tang M, et al.<\/a>; Xing et al.<\/a>; Young et al.<\/a>; Zhang T, Cui, et al.<\/a>; Zhang W, Du, et al.<\/a>). Even 12-47 days after symptom onset, feces-related air, surface, and water samples from in and around patients\u2019 toilets had SARS-CoV-2 RNA (Feng B, Xu K, et al.<\/a>). Since many patients\u2019 anal swabs test positive longer than their nasopharyngeal swabs, anal swab testing might reveal more about the patient\u2019s infection, and for deciding the length of isolation (Xiao F, Tang M, et al.<\/a>; Xu Y, Li X et al.<\/a>).<\/p>\n\n\n\n Some suspect fecal-oral transmission contributes to the epidemic, but this is unproven. (Cuicchi et al.<\/a>; de Oliveira GLV, Oliveira, et al<\/a>; Giron-Navarro et al.<\/a>; Gwenzi<\/a>; Heneghan, Spencer \u201cSARS-CoV-2 and the role of orofecal transmission\u201d<\/a>; Jones DL et al.<\/a>; Li H, Wang Y, et al.<\/a>; Mohan et al<\/a>.; Parsa et al.<\/a>; Qian Q, Fan<\/a>; Wang Y et al<\/a>; World Health Organization-China<\/a>; Wong et al.<\/a>; Xiao F, Tang M, et al.<\/a>). Almost all people defecate in toilets, so it is unlikely SARS-CoV-2 went from feces to foods. But in low income countries it could go in drinking water, aquatic foods, vegetable production, and via vectors (Gwenzi<\/a>). So, most patients probably received SARS-CoV-2 via their respiratory system, then circulatory system, then digestive system (Amirian<\/a>; Anelich et al.<\/a>; Wadman et al.<\/a>).<\/p>\n\n\n\n Some evidence shows that SARS-CoV-2 may be transmitting orally. People who took proton pump inhibitor (PPI) medicines (which reduce stomach acidity) were more likely to test positive for COVID-19 (Charpiat et al.<\/a>), or rotaviruses, influenza, norovirus, and Middle East Respiratory Virus (MERS) (Almario et al.<\/a>), than those who did not take PPI. This indirectly implies that stomach acid is protecting many people from COVID-19 and those other diseases in the gastrointestinal system. But another study did not find a correlation with PPI use (Miyake et al.<\/a>). SARS-CoV-2 has a rigid outer shell and low shell disorder, which could enable fecal-oral transmission (Gwenzi<\/a>).<\/p>\n\n\n\n SARS-CoV-2 probably can replicate in stomach cells. Scientists modeled this in vitro by generating gastric organoids (mini organs from cells in adult, pediatric, and fetal biopsies). They then infected the organoids, and cultured the resulting viruses (Giobbe et al.<\/a>).<\/p>\n\n\n\n Whether this can happen in a live person depends on if stomach acid inactivates SARS-CoV-2. The related SARS-CoV-1 is inactivated at acidic pH 1 \u2013 2 (Darnell et al.<\/a>; Scheller et al.<\/a>). SARS-CoV-2 was stable at moderate pH 3 \u2013 10, but that study did not test it at more acidic or basic pH (Chin AWH, Chu JTS, et al.<\/a>). Since these two viruses are mostly similar, SARS-CoV-2 is probably inactivated by acids. So, it probably cannot pass through the stomach gastric acid (Pressman et al.<\/a>), when it is usually pH 1.5 \u2013 3.0 (Cole, Kramer). But a heavy protein meal can neutralize stomach contents to pH 6, which might allow SARS-CoV-2 to pass (Burch<\/a>; Konturek et al.<\/a>). Scientists should conduct more experiments to find if SARS-CoV-2 is inactivated by pH<3, and by human gastric acid at its full range, 1.5 to 6. Then they could make more definite statements about whether SARS-CoV-2 can pass through the stomach and cause fecal-oral transmission.<\/p>\n\n\n\n SARS-CoV-2 could be transmitted from feces to air to people\u2019s respiratory systems (Giron-Navarro et al.<\/a>). Scientists found SARS-CoV-2 RNA in the air, toilet bowls, and surfaces in patients\u2019 bath rooms. Some found probable evidence of fecal-aerosol transmission along vertical drains through apartment bathrooms (Kang M, Wei, et al.<\/a>). So, the viruses may be coming from the patients breathing or coughing in bathrooms, or feces may be going into the air during defecation or from the swirling water while flushing (Liu Y, Deng et al.<\/a>; Liu Y, Ning, et al.<\/a>; Ong et al.<\/a>). Air or surface contamination and transmission may have occurred in villages in China and Ecuador, as shown by SARS-CoV-2 RNA in bathrooms and statistical associations between pit latrine use and SARS-CoV-2 seroconversion (Del Brutto et al. \u201cSARS-CoV-2 RNA\u201d.<\/a>; Del Brutto et al. \u201cFactors Associated\u201d<\/a>; Liu L, Hu J, et al. \u201cPit Latrines\u201d<\/a>).<\/p>\n\n\n\n To reduce transmission in bath rooms, people should ventilate and disinfect toilets and surfaces (Liu Y, Ning, et al.<\/a>; Ong et al.<\/a>), and flush with the lid down. This potential of fecal-oral transmission underscores the benefit of hand washing after defecation.<\/p>\n\n\n\n Scientists have been testing and monitoring SARS-CoV-2 RNA in wastewater to predict and prevent outbreaks (Giron-Navarro et al.<\/a>; Heneghan, Spencer \u201cSARS-CoV-2 and the role of orofecal transmission\u201d<\/a>; Koirala et al.<\/a>; Mohan et al<\/a>.; Panchal et al.<\/a>; Parida et al.<\/a>; Wolfe MK, Topol A, et al.<\/a>; Zammit et al.<\/a>). Early in the pandemic, scientists found it in wastewater and in rivers receiving wastewater in Italy, indicating that the virus might be transmitted in water, and these methods could monitor community epidemiology (Quilliam et al.<\/a>). But they could not culture the viruses, or only small amounts, showing that water might transmit the virus less than droplets, aerosols, and surfaces (Rimoldi et al.<\/a>; Zhao, Atoni, et al.<\/a>). SARS-CoV-2 can survive for days in wastewater, depending on temperature and solid contents (de Oliveira et al.<\/a>). In response to these findings, scientists in many nations are monitoring wastewater as an indicator of people\u2019s COVID-19 infections, partly using methods from the Global Polio Eradication Initiative. One study found SARS-CoV-2 in wastewater about a week after cases appeared in required weekly saliva tests (Fahrenfeld et al.<\/a>). In many areas wastewater monitoring found SARS-CoV-2 weeks before large numbers of patients in those areas developed symptoms and tested positive for SARS-CoV-2 (Baraniuk<\/a>; Harries, Berger, et al.<\/a>; Gwenzi<\/a>; Huang, Fann, et al.<\/a>; Kelland “How Sewer Science”<\/a>; Kitamura et al.<\/a>; National Academies \u201cWastewater\u201d<\/a>; O\u2019Reilly et al.<\/a>; Panchal et al.<\/a>; University of California at Merced<\/a>; Wannigama et al.<\/a>; Wurtzer S, Marechal, et al.<\/a>; Wurtzer S, Waldmann, et al.<\/a>; Wolfe MK, Topol A, et al.<\/a>; Yle<\/a>; Zhao, Atoni, et al.<\/a>). They detected SARS-CoV-2 RNA in wastewater when COVID-19 community incidence was as low as 1\/100,00 (Wolfe MK, Topol A, et al.<\/a>). Universities and schools used this method to find and stop outbreaks in dormitories and communities (Hassard et al.<\/a>; Jensen<\/a>; Nadworny<\/a>; Office of the Chancellor;<\/a> Peccia et al.<\/a>; Schmitz et al.<\/a>). Government agencies used it to monitor wastewater on airplanes from other countries, even identifying variants (Morfino, et al.<\/a>). Several nations and companies are expanding these services world-wide (IDEXX<\/a>; Munro, Colangelo<\/a>). Some wastewater programs are inactivating any SARS-CoV-2 in their systems using chemicals and ultraviolet light (Giron-Navarro et al.<\/a>; Mohan et al<\/a>.).<\/p>\n\n\n\n [Publication date of latest article cited: July 5, 2023]<\/p>\n\n\n\n SARS-CoV-2 RNA has been found in blood (World Health Organization-China<\/a>; Young et al.<\/a>; Zhang W et al.<\/a>) for an average of 16.6 days after the start of symptoms (Cevik, Tate, et al.<\/a>). ACE2 is in endothelial cells of blood vessel linings. Some authors thought that SARS-CoV-2 uses ACE2 to enter and damage those cells and tissues (Salamanna et al.<\/a>). Others thought that SARS-CoV-2 probably does not enter and multiply in the endothelium cells lining the blood vessels but it does cause dysfunction and inflammation there (Flaumenhaft et al.<\/a>). Even blood plasma from patients induces blood vessel endothelial cell activation (Karampini et al.<\/a>). SARS-CoV-2 probably goes from the vascular system into extracellular spaces, and then enters cells with much ACE2, goes to many organs and infects them, causing a wide range of symptoms. The viruses enter several bodily fluids, and then spread to other people. For example, the viruses enter the alveoli air sacs in the lungs, causing fluid increases (Flaumenhaft et al.<\/a>;Li H, Wang Y, et al.<\/a>; Wadman et al.<\/a>). This causes exhaling SARS-CoV-2, which float as aerosols. Then other people inhale them, and get infected.<\/p>\n\n\n\n But blood probably rarely transmits SARS-CoV-2 to people, because they rarely spill blood into another person\u2019s broken skin. Since RT-PCR can find almost all those infected, there is low risk of transfusion transmission (Chang L, Zhao et al.<\/a>), despite increasing prevalence in donated blood (Martinez-Acu\u00f1a et al.<\/a>). Blood banks and organ transplantation services recognized these potential risks, and screened cells, tissues, and organs before using them (Cardoso et al.<\/a>; Gaussen et al.<\/a>). No transmission by transfusion has been reported (Cappy et al.<\/a>; Berm\u00fadez-Forero et al.<\/a>; Politis et al.<\/a>; World Health Organization \u201cMaintaining a safe\u201d<\/a>). The presence of SARS-CoV-2 in blood and other body fluids enables the humoral (antibodies) and cellular (white blood cells) immune responses to attack this virus there (Allegra et al.<\/a>; Ledford<\/a>; Robbiani et al.<\/a>; Yong \u201cImmunology\u201d<\/a>).<\/p>\n\n\n\n [Publication date of latest article cited: March 23, 2023]<\/p>\n\n\n\n SARS-CoV-2 is in infected people\u2019s urine. ACE2 is expressed in kidneys (Salamanna et al.<\/a>), especially in proximal tubule (PT) cells (Lin W, Fan J, et al.<\/a>; Mumm et al.<\/a>), and in prostate tubules and bladders (Mumm et al.<\/a>). Many COVID-19 patients develop acute kidney injury (Hansrivijit et al.<\/a>). SARS-CoV-2 was found in some people’s urine (de Mattos et al.<\/a>; Jeong et al<\/a>; Jones DL. et al.<\/a>; Karia et al.<\/a>; Peng et al.<\/a>; Saylam et al.<\/a>; Sun J Zhu A, et al.<\/a>), and was cultured in cells (Jeong et al<\/a>; Sun et al.<\/a>). It was not detected in any infected people\u2019s urine in several other studies (Lo IL, Lio, et al.<\/a>; To, Tsang KKW, Leung et al.<\/a>; Wang W, Xu, et al<\/a>; Young et al.<\/a>). Tests found SARS-CoV-2 in urine of 2% \u2013 6.9% of patients who had these viruses in other body fluids (Bronimann et al.<\/a>; Garcia-Bernalt et al.<\/a>).<\/p>\n\n\n\n These viruses could be transmitting in people\u2019s urine (Parida et al.<\/a>). Scientists inoculated ferrets nasally with COVID-19 patients\u2019 urine, resulting in infection (Jeong et al<\/a>). SARS-CoV-2 RNA was found on toilet seats and flush handles (Amoah et al.<\/a>). Experiments found that flushing urinals (Wang JX, Li YY, et al.<\/a>) and toilets (Crimaldi et al.<\/a>; Li YY, Wang, et al.<\/a>) sprays a plume of microscopic liquid particles into the air. Perhaps some people could get infected from breathing in restrooms. Two cases may have been infected from a public toilet (Global Times<\/a>). But COVID-19 patients more often had SARS-CoV-2 in their nasopharyngeal swabs than in urine, showing that urine is probably not one of the main transmission sources (Mumm et al.<\/a>).<\/p>\n\n\n\n People could reduce the probability of transmission via urine by disinfecting toilets and urinals (Abney et al.<\/a>; Amoah et al<\/a>.), and wearing masks in public bathrooms (Wang JX, Li YY, et al.<\/a>). SARS-CoV-2 in urine could also be aerosolized during laboratory procedures, such as centrifuging. So, laboratory staff should add more safety methods, including wearing face masks, face shields, and using containment devices (de Mattos et al.<\/a>), or should avoid doing microscopy of urine (Hansrivijit et al.<\/a>).<\/p>\n","protected":false},"excerpt":{"rendered":" Saliva and mucous droplets [Publication date of latest article cited: October 16, 2023] Saliva and mucous, and the tissues producing them, are some of the main routes in which SARS-CoV-2 replicates and transmits (Collins; Huang N, Perez, et al.; Li H, Wang Y, et al.; Nazaroff; Wang C, Prather K, et al.). SARS-CoV-2 replicates in […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-73","page","type-page","status-publish","hentry","post"],"_links":{"self":[{"href":"https:\/\/vryheid.sdsu.edu\/index.php\/wp-json\/wp\/v2\/pages\/73","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vryheid.sdsu.edu\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/vryheid.sdsu.edu\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/vryheid.sdsu.edu\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vryheid.sdsu.edu\/index.php\/wp-json\/wp\/v2\/comments?post=73"}],"version-history":[{"count":30,"href":"https:\/\/vryheid.sdsu.edu\/index.php\/wp-json\/wp\/v2\/pages\/73\/revisions"}],"predecessor-version":[{"id":2934,"href":"https:\/\/vryheid.sdsu.edu\/index.php\/wp-json\/wp\/v2\/pages\/73\/revisions\/2934"}],"wp:attachment":[{"href":"https:\/\/vryheid.sdsu.edu\/index.php\/wp-json\/wp\/v2\/media?parent=73"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}Aerosols<\/h2>\n\n\n\n
Feces<\/h2>\n\n\n\n
Blood<\/h2>\n\n\n\n
Urine<\/h2>\n\n\n\n